Sakura Matō (間桐 桜, Matō Sakura
?)
A student in her first year of high school, and the sister of Shinji Matō. After Shirō's father Kiritsugu died, Sakura often visited Shirō's home to help him with his daily chores. Though Shinji is from a family of sorcerers, he asserts that she has no knowledge of her family's craft or history. Sakura is outwardly shy and timid, but possesses great inner strength. She has a long standing and obvious crush on Shirō Emiya.
Kirei Kotomine (言峰 綺礼, Kotomine Kirei
?)
Through an agreement between the Mages' Association and the Church, the Holy Grail Wars are moderated by a priest. Kirei's father was the mediator for the fourth Holy Grail War, and when he died, Kirei felt obligated to replace him. His selection as mediator is unusual, for Kirei is a member of both the Church and Mages' Association, organizations on hostile terms with one another. Since the death of Rin's father, he has acted as an indirect caretaker for Rin. Ranked far lower in the Church hierarchy than(from Tsukihime), Kirei employs many of the same techniques and weapons she does.
Lancer (ランサー, Ransā
?)
The first hostile Servant to appear in the story. Lancer is a fierce but balanced warrior who enjoys combating a worthy opponent. He has a playful attitude and takes a very carefree approach to life, but is quick to work himself into a frenzy during a heated battle. Lancers are hailed as the "Most Agile" of Servant classes.
Kiritsugu Emiya (衛宮切嗣, Emiya Kiritsugu
?)
Shirō's foster father, and his savior from the aftermath of the massive Fuyuki fire. During his lifetime, Kiritsugu strove to be a Hero of Justice, a defender of humanity who could protect the weak. He feels he failed to do so, but his efforts were a source of inspiration for Shirō. Kiritsugu was a sorcerer, though he never taught Shirō seriously and Shirō had little talent for the craft. He died several years prior to the beginning of Fate/stay night from an unknown cause.
Taiga Fujimura (藤村 大河, Fujimura Taiga
?)
She is an English teacher at Shirō's school, homeroom instructor of Shirō's class and the supervising teacher for the archery dojo. She is widely called "Tiger" (タイガー, Taigā
?), a nickname she dislikes ("Taiga" and "Tiger" sound similar in Japanese), though Shirō refers to her as "Fuji-nee" (older sister Fuji). After Kiritsugu died, Taiga became Shirō's guardian and has been living with him for several years. Taiga and Shirō are very close, and she regards him as a younger brother.
Gilgamesh (ギルガメッシュ, Girugamesshu
?)
An arrogant, selfish, and enigmatic Archer-class Servant. He appears familiar with Fuyuki City and claims to have a past relationship with Saber. Typically clad in an ornate golden armor, he possesses an immense number of Noble Phantasms, though none are representative of his true identity.
Sōichirō Kuzuki (葛木 宗一郎, Kuzuki Sōichirō
?)
A well-built man with angular glasses and a conservative hairstyle. He is the strict homeroom instructor of Rin's class. He primarily lectures on History, though he holds classes in ethics. Sōichirō is well respected among staff and students, but he is widely regarded as austere and impersonal.
Caster (キャスター, Kyasutā
?)
She is an honored guest of the Ryūdō family, permitted to live at their temple until the preparations for her marriage have been finalized. A gorgeous and talented yet mysterious woman of high stature, her presence has attracted the attention of trainee priests.
Assassin (アサシン, Asashin
?)
A solitary Servant, clothed in a traditional Japanese hakama and kimono with an indigo haori. This handsome man bars entry beyond the main gate of the Ryūdōji Temple. Quiet and composed, he maintains a degree of bushido and is eager to spar with other Servants. He is of the same class, but he is not to be confused with another Servant known as True Assassin.